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排序方式: 共有146条查询结果,搜索用时 15 毫秒
1.
The observation that a fraction of organic matter produced in marine systems evades the concerted efforts of microbial communities and is buried in sediments suggests that there are ‘speed bumps’ in carbon degradation pathways that impede microbially driven remineralization processes. The initial step in degradation of macromolecules, extracellular enzymatic hydrolysis, is often stated to be ‘the’ rate-limiting step in carbon remineralization. Experimental investigations described here, however, demonstrate that at least in certain cases, microbes produce extracellular enzymes on time scales of hours to tens of hours in response to substrate addition, and hydrolysis is extremely rapid. If enzymatic hydrolysis can be rapid, what factors slow or stop organic matter degradation? A lack of the correct inducer to initiate enzyme production, and/or a lack of the correct organism to produce the required enzyme, may result in a complete lack of hydrolysis in certain environments—a barricade, rather than a speed bump. Preliminary evidence supporting this hypothesis includes a comparison of polysaccharide hydrolysis in seawater and sediments, which demonstrates that the spectrum of enzymes active in seawater and sediments are fundamentally different. Furthermore, a survey of enzyme activities in surface waters from a range of locations suggests that pelagic microbial communities also differ widely in their abilities to express specific extracellular enzymes. Trans-membrane transport through porins is yet another potential location of structure-related selectivity.Our efforts to identify speed bumps and barricades are hampered by our inability to structurally characterize in sufficient detail the macromolecular structures present in marine systems. Furthermore, assessments of organic matter ‘quality’ from a chemical perspective do not necessarily accurately reflect the availability of organic carbon to microbial communities. For these communities, in fact, ‘quality’ may be a variable, which depends on the enzymatic and uptake capabilities of community members. To begin to assess substrate structure and quality from a microbial perspective, we will have to combine specific knowledge of macromolecular structures with detailed investigations of the enzymatic and transport capabilities of heterotrophic marine microbes. 相似文献
2.
探讨了用复合酶法制备鱼是鱼活性寡肽及其氨基酸组成和相对分子质量分布情况。应用正交试验法研究了酶量、酶解温度、酶解时间对鱼是鱼蛋白质水解度和酶解液苦味的影响。结果表明,最佳酶解作用条件是:酶量为100u/mL;酶解温度为50℃;酶解时间为4h。此时的酶解液苦味较低,水解度可达62.81%。应用氨基酸分析仪和凝胶排阻高效液相色谱分析了该寡肽的氨基酸组成和相对分子质量的分布范围,其十肽以下的小肽占67.77%,氨基酸组成平衡,必需氨基酸含量丰富。鱼是鱼活性寡肽有望成为保健食品、医药等领域的新型高品质原料。 相似文献
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4.
采用硫酸铵分部沉淀与凝胶过滤的方法,进行藓羽藻Rubisco的分离研究。结果表明,分离的藓羽藻Rubisco经SDS-聚丙烯酰胺凝胶电泳检测呈两条清晰条带,分别为Rubisco大亚基与小亚基;与菠菜相比,藓羽藻Rubisco大亚基分子量与菠菜基本相同,而小亚基较之稍大一些。藓羽藻Rubisco活力测定结果表明,Rubisco分离过程中用硫酸铵分部沉淀后活力降低许多,分离后活力有所上升,但仍比粗提液活力弱;在Rubisco活力测定过程中,藓羽藻Rubisco的活化温度与其它物种Rubisco活化的温度不同,在低温下活化效果较好。这些结果说明Rubisco的酶活力受硫酸铵的影响而且藓羽藻Rubisco相对陆地高等植物结构不稳定。 相似文献
5.
The Removal of Dissolved Metals by Hydroxysulphate Precipitates during Oxidation and Neutralization of Acid Mine Waters, Iberian Pyrite Belt 总被引:4,自引:0,他引:4
J. Sánchez España E. López Pamo E. Santofimia Pastor J. Reyes Andrés J. A. Martín Rubí 《Aquatic Geochemistry》2006,12(3):269-298
This study examines the removal of dissolved metals during the oxidation and neutralization of five acid mine drainage (AMD)
waters from La Zarza, Lomero, Esperanza, Corta Atalaya and Poderosa mines (Iberian Pyrite Belt, Huelva, Spain). These waters
were selected to cover the spectrum of pH (2.2–3.5) and chemical composition (e.g., 319–2,103 mg/L Fe; 2.85–33.3 g/L SO4=) of the IPB mine waters. The experiments were conducted in the laboratory to simulate the geochemical evolution previously
recognized in the field. This evolution includes two stages: (1) oxidation of dissolved Fe(II) followed by hydrolysis and
precipitation of Fe(III), and (2) progressive pH increase during mixing with fresh waters. Fe(III) precipitates at pH < 3.5
(stages 1 and 2) in the form of schwertmannite, whereas Al precipitates during stage 2 at pH 5.0 in the form of several hydroxysulphates
of variable composition (hydrobasaluminite, basaluminite, aluminite). During these stages, trace elements are totally or partially
sorbed and/or coprecipitated at different rates depending basically on pH, as well as on the activity of the SO4=
anion (which determines the speciation of metals). The general trend for the metals which are chiefly present as aqueous
free cations (Pb2+, Zn2+, Cu2+, Cd2+, Mn2+, Co2+, Ni2+) is a progressive sorption at increasing pH. On the other hand, As and V (mainly present as anionic species) are completely
scavenged during the oxidation stage at pH < 3.5. In waters with high activities (> 10−1) of the SO 4= ion, some elements like Al, Zn, Cd, Pb and U can also form anionic bisulphate complexes and be significantly sorbed at pH
< 5. The removal rates at pH 7.0 range from around 100% for As, V, Cu and U, and 60–80% for Pb, to less than 20% for Zn, Co,
Ni and Mn. These processes of metal removal represent a significant mechanism of natural attenuation in the IPB. 相似文献
6.
Organic pollutants associated with macromolecular soil organic matter: Mode of binding 总被引:2,自引:0,他引:2
H. H. Richnow R. Seifert J. Hefter M. Link W. Francke G. Schaefer W. Michaelis 《Organic Geochemistry》1997,26(11-12)
A study of ether-linked moieties in macromolecular bound residues of polycyclic aromatic hydrocarbons (PAH) generated in bioremediation experiments was performed using high temperature hydrolysis degradation with subsequent analysis of the products by GC-MS. This hydrolysis reaction was specifically designed to cleave ether bonds including relatively stable diarylether structures. Among the reaction products, aromatic alcohols representing typical microbiologically derived metabolites of PAH were found in addition to natural compounds. Thus, parts of the bound residues appeared to be linked within the macromolecular material by ether bonds. Model experiments with an oxidoreductase enzyme and aromatic alcohols indicate the formation of these ether bonds to be an enzyme-catalysed process. 相似文献
7.
A Competitive Immunoassay for the Determination of the Herbicide Fluazifop in Drinking Water and Groundwater A competitive solid-phase enzyme immunoassay using rabbit polyclonal antibodies was developed for the detection of the herbicide fluazifop [(RS)-2-[4-(5-trifluoromethyl-2-pyridyloxy)phenoxy]propionic acid] in drinking water and groundwater. Present regulatory limits for drinking water in Germany were taken as the critical level. The carrier protein was bovine serum albumin; horseradish peroxidase was used as marker enzyme with 3,3′,5,5′-tetramethylbenzidine as substrate. A high concentration of high-affinity antibodies in the serum, optimization of test conditions (antibody and enzyme tracer concentration, incubation time etc.), and very low cross reactivities to substances of similar structures led to a highly sensitive and specific ELISA with a detection limit below 0.1 μg/L for fluazifop as free acid. On testing the suitability of the assay's use as a screening test with one hundred drinking-water samples, the three samples which had been spiked in the laboratory were recognized as positive with respect to their fluazifop content. Confirmation by gas chromatography-mass spectrometry showed the test results of two other samples to be false positive. False negative results did not appear. The concentration was in the detection limit region of 0.1 μg/L. 相似文献
8.
9.
A highly sensitive and specific enzyme immunoassay (EIA) is described for the detection of the atrazine metabolite hydroxyatrazine. Polyclonal antibodies were raised in rabbits by immunization with a hapten-bovine serum albumin (BSA) conjugate containing 8 hapten residues per molecule of BSA. An EIA with a horseradish peroxidase (HRP) hapten tracer was optimized in microtitre plates. A concentration of 50% B/B0 was found at 0.10 μg/L for hydroxyatrazine. A limit of determination for hydroxyatrazine was reached at approximately 0.01 μg/L, i.e. well below the maximum concentration permitted by the EU guidelines for drinking water and the drinking water ordinance of the FRG. The assay did not require concentration or clean-up steps for drinking water or ground water samples. Validation experiments confirmed a good accuracy and precision. Hydroxyatrazine is reported to be the main atrazine metabolite found in soil samples. As organic solvents are usually employed for soil extraction, the influence of methanol as representative organic solvent on the assay was examined. Up to a concentration of 5% (v/v) methanol, the organic solvent did not affect the assay. 相似文献
10.
A screening method for heavy metals in aqueous extracts of soil is presented which is based on inhibition of the enzymes urease and alcohol dehydrogenase. The method is suitable to detect cupric and mercury ions in concentrations below 0.01 mg/L and several other heavy metal ions in 1000 fold higher concentration. It is shown that the test may be used for screening of mercury ion concentrations exceeding 0.03 mg/L in aqueous solution when copper chelators are added to the test system. The usefulness of the presented tests to detect heavy metals eluted from soil was verified with samples from ore mining waste. The concentration of copper, lead, and zinc eluted from these samples to different amount was determined by atomic absorption spectrometry and was in good agreement with the enzyme inhibition data obtained with these samples. 相似文献